nELISA: True Multiplexing at Scale
What if simple refinements to the age-old ELISA could have profound impacts on its scalability?
see how it works
nELISA WORKFLOW
Surface nano-architecture
At the surface of every bead, pairs of target-specific antibodies are pre-assembled, before the assay even begins. Capture antibodies are immobilized, while detection antibodies are flexibly and reversibly tethered to the surface using a DNA construct.
nELISA WORKFLOW
Target Co-Recognition
If a target protein is present, it is recognized by both the capture and detection antibodies, forming a sandwich complex. The detection antibody is now bound-back to the bead surface.
nELISA WORKFLOW
Detection-by-Displacement
A fluorescently-labeled displacer oligo is added to each well, releasing the detection antibody through a DNA displacement reaction. If the antibody is bound to its target, it stays attached to the bead, resulting in the labeling of the bead with a fluorescent dye.
nELISA WORKFLOW
No Cross-Reactivity
When non-specific interactions occur with either antibody, the sandwich complex fails to form. The detection antibody rinses off, taking the fluorescently labeled oligo along with it. With that, an nELISA bead only yields a signal in the presence of its target.
The nELISA Technology
Three strategies put together to bring about step-function change.
Colocalization for True Multiplexing
Using DNA as a flexible, programmable linker to completely overcome cross-reactivity.
Traditional Multiplexing
Mixing antibodies in traditional multiplexed ELISAs leads to spurious cross-reactivity.
Proximity-based Multiplexing
Using complementary strands with PCR or NGS to minimize cross-reactivity, after it has occured.
Colocalization by nELISA
Using universal DNA structures to pre-assemble antibody pairs on their cognate beads prevents cross-reactivity from occurring in the first place. That way, the signal is never impacted by the level of multiplexing, achieving True Multiplexing.
Next-Level Fluorescent Barcoding
The power of combinatorial spectral multiplexing unlocked using nano-manufacturing and physical models.
4
dyes
>3,000
spectral barcodes
High-throughput, simple workflow
>10,000 samples per week using a surprisingly simple workflow - all using commercially available instrumentation.
nELISA Resources
Check out these resources to dive into the core technology and its applications.
Learn about nELISA targets
Towards proteome-wide interrogation, we’ve started with the inflammatory secretome. See what targets we’ve on-boarded to-date, what targets are coming up next, and how you can add your own on the Targets page
